Use of reflectance spectrophotometry and colorimetry in a general linear model for the determination of the age of bruises
Monday, June 21st, 2010This study sought to determine
if colorimetry or reflectance spectrophotometry could be employed to objectively estimate the age of bruises. Based on a previously
described method, reflectance spectrophotometric scans were obtained from bruises using a Cary 100 Bio spectrophotometer fitted
with a fibre-optic reflectance probe. Measurements were taken from the bruise and a control area. Software was used to calculate
the first derivative at 490 and 480 nm; the proportion of oxygenated hemoglobin was calculated using an isobestic point method
and a software application converted the scan data into colorimetry data. In addition, data on factors that might be associated
with the determination of the age of a bruise: subject age, subject sex, degree of trauma, bruise size, skin color...
