8-hydroxycannabinol: a new metabolite of cannabinol formed by human hepatic microsomes
Abstract Metabolism of cannabinol (CBN) was studied in vitro using hepatic microsomes from human livers. The metabolites formed were
analyzed by thinlayer chromatography (TLC) and identified by gas chromatography-mass spectrometry as their trimethylsilyl
derivatives. 11-Hydroxy-CBN, the major metabolite, was detected together with a smaller amount of another mono-hydroxylated
metabolite. The minor metabolite was identified as 8-hydroxy-CBN, after comparing its Rf value by TLC, retention time by GC,
and the mass spectrum with those of the authentic compound. 8-Hydroxy-CBN was confirmed to be a new metabolite of CBN formed
by human hepatic microsomes.
Content TypeJournal Article
JournalForensic ToxicologyOnline ISSN 1860-8973Print ISSN 1860-8965 (Source: Forensic Toxicology)
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