As busy as September is, with some work for a course on the University of Amsterdam, for the union, for preparing the ENFSI Forensic IT working group meeting www.enfsi.org , organzing some conference and writing for a book.These months there were some ...
Abstract A fully automated method for analysis of amphetamine-related drugs in human hair by gas chromatography-mass spectrometry (GC-MS)
was developed using headspace solid-phase microextraction (SPME) and in-matrix derivatization. Amphetamines were extracted
from hair under alkaline conditions, and were simultaneously derivatized to N-ethoxycarbonyl amphetamines with ethylchloroformate in a vial. An SPME fiber was then exposed to the headspace at 80°C for
10 min for extraction. The derivatives extracted into the stationary phase of the fiber were desorbed by exposing the fiber
in an injection port of a GC-MS instrument. The calibration curves showed linearity up to 10ng/mg in hair. The detection limits
ranged from 0.01 to 0.5 ng/mg according to the compound identity. No interferences were found, and the time required for analysis
was about 30 min per sample. Furthermore, this proposed method was applied to diagnosis of methamphetamine intake in actual
cases; methamphetamine and its metabolite amphetamine were able to be detected in hair of abuser patients admitted to a hospital.
Content TypeJournal Article
JournalForensic ToxicologyOnline ISSN 1860-8973Print ISSN 1860-8965 (Source: Forensic Toxicology)
Abstract A rapid and simple method for determination of oxazolobenzodiazepines (oxazolam, haloxazolam, mexazolam, cloxazolam, and flutazolam)
in sera was developed by gas chromatography (GC) using nitrogen-phosphorus detection (NPD) and a methyl silicone fused-silica
wide-bore capillary (DB-1) column. Underivatized oxazolobenzodiazepine drugs gave relatively low sensitivity, because of decomposition
during their passage through the column. On the other hand, on-column methylation of the oxazolobenzodiazepines with 0.4 mM
trimethylanilium hydroxide in methanolic solution resulted in higher sensitivity; detection was possible at levels at least
two to eight times lower than those without derivatization. The average recoveries of five oxazolobenzodiazepines from 0.5-ml
volumes of sera containing 1.0 or 0.1 μg of each drug were 88–101% and 66–106%, respectively. The limits of detection of methylated
oxazolobenzodiazepines by wide-bore capillary GC-NPD were 50–100pg on column. This method could be used for determination
of the drugs in actual serum specimens with concentrations as low as 25–70ng/ml.
Content TypeJournal Article
JournalForensic ToxicologyOnline ISSN 1860-8973Print ISSN 1860-8965 (Source: Forensic Toxicology)
